Journal: Cell Reports Medicine

Article Title: Macrophage-mimetic photothermal nanotherapeutics regulate mitochondrial homeostasis and inflammatory cascades in lung ischemia-reperfusion injury

doi: 10.1016/j.xcrm.2026.102768

Figure Lengend Snippet: Rg3@PACVs attenuate oxidative stress and mitochondrial dysfunction in hypoxia/reoxygenation (H/R)-injured lung epithelial cells (A) Reactive oxygen species (ROS) levels detected by flow cytometry using DCFH-DA probe. (B) Intracellular total superoxide dismutase (T-SOD) activity. (C) Malondialdehyde (MDA) content. (D) Glutathione/oxidized glutathione (GSH/GSSG) ratio. (E) Immunofluorescence detection of nuclear-factor-erythroid-2-related factor 2 (NRF-2, green) and heme oxygenase-1 (HO-1, red). Nuclei counterstained with DAPI (blue). Scale bars, 100 μm. (F) Mitochondrial membrane potential assessed by JC-1 staining. (G) Super-resolution microscopy of mitochondrial ultrastructure (red) in epithelial cells. Lower panel shows magnified views (∗ represents damaged mitochondria, Scale bars, 5 μm). (H–L) Mitochondrial respiration in BEAS-2B cells under different stimulation conditions was assessed by measuring the oxygen consumption rate (OCR) using a Seahorse XF96 analyzer (H). Key parameters including basal respiration (I), maximal respiration (J), proton leak (K), and ATP production (L) were calculated. Data are presented as the mean ± SD and analyzed using one-way ANOVA with Tukey’s post hoc test ( ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. H/R; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. H/R + Rg3@PACVs; n = 4 biological replicates).

Article Snippet: For adherent cells, coverslips were fixed with 4% paraformaldehyde after treatment, blocked with 5% bovine serum albumin (BSA, Coolaber, China) for 30 min, and incubated with primary antibodies: NRF-2 ( GB113808 , Servicebio), HO-1 ( GB115713 , Servicebio), NLRP3 ( GB114320 , Servicebio), COX-2 ( GB155672 , Servicebio), ZO-1 (GB15195, Servicebio), E-cadherin (GB11082, Servicebio), HMGB1 (GB11103, Servicebio), CD11b (GB15058, Servicebio), and MPO ( GB150006 , Servicebio).

Techniques: Flow Cytometry, Activity Assay, Immunofluorescence, Membrane, Staining, Super-Resolution Microscopy, Control

Journal: Metabolic Brain Disease

Article Title: The SIRT-1/Nrf-2/HO-1 antioxidant defense axis in adult attention-deficit/hyperactivity disorder

doi: 10.1007/s11011-026-01845-5

Figure Lengend Snippet: ROC Curve Analysis for HO-1, Nrf-2 and SIRT-1. HO-1 (cut-off value 0.87, sensitivity 78.3%, specificity 76.7%); Nrf-2 (cut-off value 9.8 sensitivity 70%, specificity 73.3%); SIRT-1 (cut-off value 8.3, sensitivity 75%, specificity 70%). Abbreviations: HO-1: heme oxygenases-1; Nrf-2: nuclear factor erythroid 2-related factor 2; SIRT-1: Sirtuin 1

Article Snippet: Serum HO-1, NRF-2, and SIRT-1 concentrations were analyzed using ELISA kits according to the manufacturers’ standard protocols (BT Lab, Human Heme Oxygenase-1: Cat. No. E0932Hu; BT Lab, Human Nuclear Factor Erythroid 2-Related Factor 2: Cat. No. E3244Hu; BT Lab, Human Sirtuin-1: Cat. No. E2557Hu; Jiaxing Korain Biotech, Jiaxing, China) on a Rel Assay automated ELISA reader (Biobase Biodusty Co., Ltd., Jinan, China).

Techniques:

Journal: iScience

Article Title: USP1 promotes hepatocellular carcinoma progression by modulating mitophagy via stabilizing MCM3 to regulate the Keap1-Nrf2 axis

doi: 10.1016/j.isci.2026.114927

Figure Lengend Snippet: MCM3 regulates mitophagy by influencing the expression and localization of Nrf-2 (A) WB used to detect the impact of MCM3 knockdown on P62, LC3B II/I, TOMM20, FUNDC1, Parkin, and PINK1 in Huh7 and LM3 cells. (B) WB was used to assess the impact of MCM3 knockdown on Nucleus Nrf2 in Huh7 and LM3 cells. (C) IF was used to evaluate the impact of MCM3 knockdown on Nrf2 in Huh7 and LM3 cells ( n = 3 biologically independent experiments). (D) WB was used to measure the impact of MCM3 knockdown on HMOX-1 and NQO1 expression in Huh7 and LM3 cells, with or without THBQ. (E) WB was performed to detect the impact of MCM3 knockdown on P62, LC3B II/I, TOMM20, FUNDC1, Parkin, and PINK1 in Huh7 and LM3 cells, with or without THBQ. Data are presented as mean ± SD. ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05, ns p ≥ 0.05. For (A), (D), and (E), statistical analysis was performed using a one-way ANOVA ( n = 3 biologically independent experiments); For B, statistical analysis was performed using a two-tailed unpaired Student’s t test ( n = 3 biologically independent experiments).

Article Snippet: Following three washes with PBS, the cells were blocked with an immunofluorescence blocking solution (Beyotime) at 37°C for 1 h. Subsequently, the cells were incubated with a primary antibody against Nrf-2 (Proteintech, #16396-1-AP, 1:50 dilution) at 4°C overnight.

Techniques: Expressing, Knockdown, Two Tailed Test

Journal: iScience

Article Title: USP1 promotes hepatocellular carcinoma progression by modulating mitophagy via stabilizing MCM3 to regulate the Keap1-Nrf2 axis

doi: 10.1016/j.isci.2026.114927

Figure Lengend Snippet: MCM3 regulates mitophagy by influencing the expression and localization of Nrf-2 (A) WB used to detect the impact of MCM3 knockdown on P62, LC3B II/I, TOMM20, FUNDC1, Parkin, and PINK1 in Huh7 and LM3 cells. (B) WB was used to assess the impact of MCM3 knockdown on Nucleus Nrf2 in Huh7 and LM3 cells. (C) IF was used to evaluate the impact of MCM3 knockdown on Nrf2 in Huh7 and LM3 cells ( n = 3 biologically independent experiments). (D) WB was used to measure the impact of MCM3 knockdown on HMOX-1 and NQO1 expression in Huh7 and LM3 cells, with or without THBQ. (E) WB was performed to detect the impact of MCM3 knockdown on P62, LC3B II/I, TOMM20, FUNDC1, Parkin, and PINK1 in Huh7 and LM3 cells, with or without THBQ. Data are presented as mean ± SD. ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05, ns p ≥ 0.05. For (A), (D), and (E), statistical analysis was performed using a one-way ANOVA ( n = 3 biologically independent experiments); For B, statistical analysis was performed using a two-tailed unpaired Student’s t test ( n = 3 biologically independent experiments).

Article Snippet: Nrf-2 , Proteintech , Cat# 16396-1-AP; RRID: AB_2782956.

Techniques: Expressing, Knockdown, Two Tailed Test